Coding
CCBD
Part:BBa_M45405:Design
Designed by: Farhad Farjood Group: UtahState_BE5930_-_S14 (2015-04-10)
Human Fibronectin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 260
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The amino acid sequence is back-translated to DNA sequence which is codon-optimized for Homo sapiens
Note: There are two changes noted on the DNA. These changes were made to get rid of restriction sites for AgeI. If this change isn't needed then the sequence can be changed back to ACCGGT at these sites. The changes are annotated on the DNA sequence.
Source
Homo sapiens